Professor, School of Life Sciences

University of Science and Technology of China

443 Huangshan Street

Hefei, Anhui 230027

P. R. China

Email: dlong[at]ustc.edu.cn

Personal Profile:

B.Sc.  (2005)  Wuhan University, Wuhan, Hubei, China  

Ph.D.  (2010)  National University of Singapore, Singapore

2010 - 2012, Postdoctoral Fellow, Florida State University/National High Magnetic Field Laboratory, Tallahassee, Florida, United States

2012 - 2015, Postdoctoral Fellow, University of Toronto, Toronto, ON, Canada

2015 - present, Professor, University of Science and Technology of China, Hefei, China

Research Interest

• Investigation of the structures and dynamical properties of functionally important proteins and other macromolecules using NMR spectroscopy;

• Computational modeling/simulation of the structure and dynamics of macromolecular systems;

• Development of NMR and computational methodology for structural and dynamics studies of biomacromolecules.

Selected Publications:

1) D. Liu, Y. Mao, X. Gu, Y. Zhou, D. Long* (2021) Unveiling the invisible druggable conformations of GDP-bound inactive Ras. Proc. Natl. Acad. Sci. U.S.A. 118(11):e2024725118

2) X. Chen, H. Gao, D. Long* (2021) Millisecond Allosteric Dynamics of Activated Ras Reproduced with a Slowly Hydrolyzable GTP Analogue. ChemBioChem 22:1079-1083

3) D. Liu, X. Chen, D. Long* (2020) NMR-Derived Conformational Ensemble of State 1 of Activated Ras Reveals Insights into a Druggable Pocket. J. Phys. Chem. Lett. 11:3642-3646

4) X. Chen, H. Yao, H. Wang, Y. Mao, D. Liu, D. Long* (2019) Extending the lifetime of native GTP-bound Ras for site-resolved NMR measurements: Quantifying the allosteric dynamics. Angew. Chem. Int. Ed. 58:2730-2733

5) P. Cheng, D. Liu, P. X. Chee, D. Yang, D. Long* (2019) Atomistic Insights into the Functional Instability of the Second Helix of Fatty Acid Binding Protein. Biophys. J. 117:239-246

6) S. Li, R. Hu, H. Yao, D. Long, F. Luo, X. Zhou, X. Zhang, M. Liu, J. Zhu, Y. Yang (2018) Characterization of the interaction interface and conformational dynamics of human TGIF1 homeodomain upon the binding of consensus DNA. BBA - Proteins Proteom. 1866:1021-1028

7) H. Wang, S. Wang, C. Li, H. Li, Y. Mao, W. Liu, C. Xu*, D. Long* (2017) Probing transient release of membrane-sequestered tyrosine-based signaling motif by solution NMR spectroscopy. J. Phys. Chem. Lett. 8:3765-3769

8) Y. Mao, H. Yao, H. Wang, P. Cheng, D. Long* (2016) Microsecond timescale dynamics of GDP-bound Ras underlies the formation of novel inhibitor-binding pockets. Angew. Chem. Int. Ed. 55:15629-15632

9) D. Long,* F. Delaglio, A. Sekhar, L. E. Kay* (2015) Probing invisible, excited protein states by non-uniformly sampled pseudo-4D CEST spectroscopy. Angew. Chem. Int. Ed. 54:10507-10511

10) D. Long, A. Sekhar, L. E. Kay (2014) Triple resonance-based ¹³Cα and ¹³Cβ CEST experiments for studies of ms timescale dynamics in proteins. J. Biomol. NMR 60:203-208

11) D. Long, G. Bouvignies, L. E. Kay (2014) Measuring hydrogen exchange rates in invisible protein excited states. Proc. Natl. Acad. Sci. U.S.A. 111:8820-8825

12) D. Long, C. B. Marshall, G. Bouvignies, M. T. Mazhab-Jafari, M. J. Smith, M. Ikura, L. E. Kay. (2013) A comparative CEST NMR study of slow conformational dynamics of small GTPases complexed with GTP and GTP analogues. Angew. Chem. Int. Ed. 52:10771-10774

13) H. Sun, D. Long, R. Brüschweiler, V. Tugarinov. (2013) Carbon relaxation in ¹³Cα-Hα and ¹³Cα-Dα spin pairs as a probe of backbone dynamics in proteins. J. Phys. Chem. B 117:1308-1320

14) D. Long, R. Brüschweiler. (2013) Directional selection precedes conformational selection in ubiquitin-UIM binding. Angew. Chem. Int. Ed. 52: 3709-3711

15) D. Long, R. Brüschweiler. (2012) Structural and entropic allosteric signal transduction strength via correlated motions. J. Phys. Chem. Lett. 3:1722-1726

16) D. Long, R. Brüschweiler. (2011) Atomistic kinetic model for population shift and allostery in biomolecules. J. Am. Chem. Soc. 133:18999-19005

17) D. Long, D.W. Li, K. Walter, C. Griesinger, R. Brüschweiler. (2011) Toward a predictive understanding of slow methyl group dynamics in proteins. Biophys. J. 101:910-915

18) D. Long, R. Brüschweiler. (2011) In silico elucidation of the recognition dynamics of ubiquitin. PLoS Comput. Biol. 7: e1002035