Main Research Directions
Development and biomedical applications of functionalized cells and protein probes:
1. Develop novel protein probes using genetic code expansion and bioorthogonal chemistry to reveal the activity and physiological functions of protein enzymes and post-translational modifications.
2. Develop new screening and evolution techniques to modify enzyme activity and function.
3. Construct novel functionalized cells and develop new therapies targeting cancer and pathogens.
Position
Special Professor and PhD Supervisor at the School of Life Sciences and Medicine, University of Science and Technology of China. Awarded the 2022 National Young Overseas High-Level Talent Program.
Education
- Bachelor’s degree in Chemistry from Tsinghua University (2007-2011)
- PhD in Chemistry from Tsinghua University (2011-2016)
Work Experience
- Postdoctoral Researcher at the MRC Laboratory of Molecular Biology, UK (2016-2022)
- Special Professor at the University of Science and Technology of China (2022-present)
Achievements
Published over ten papers as first (including co-first) and co-corresponding author in journals such as Nature, Nature Protocols, Nature Communications, Science Advances, PNAS, Angew Chem, Chem Sci and Acc Chem Res. Received the EMBO Long-Term Fellowship (2017-2018) and was a Postdoctoral Research Associate at Queen’s College, University of Cambridge (2018-2020). Joined the School of Life Sciences and Medicine at USTC in July 2022, focusing on using chemical biology to study the functions of protein enzymes and post-translational modifications in vivo and their roles in diseases. Also develops new functional enzymes, functionalized cells, and protein molecules for diagnosing and treating cancer and infectious diseases through synthetic biology.
Publications
(1) Dong-Liang Huang, Wu-Chen Guo, Wei-Wei Shi, Yun-Pu Gao, Yong-Kang Zhou, Long-Jie Wang, Chen Wang, Shan Tang,* Lei Liu,* Ji-Shen Zheng.* Enhanced native chemical ligation by peptide conjugation in trifluoroacetic acid. Sci. Adv. 2024, 10, eado9413. (Co-corresponding)
(2) Juan Luo, Yao Yu, Ke Wang, Sizhe He, Longjie Wang, Fangfang Liang, Jason W. Chin, Shan Tang.* Capturing acyl-enzyme intermediates with genetically encoded 2,3-diaminoproionic acid for hydrolase substrate identification. Nat. Protoc. 2024, doi.org/10.1038/s41596-024-01006-x. (Corresponding)
(3) Yun-Pu Gao, Peng-Fei Sun, Wu-Chen Guo, Yong-Kang Zhou, Ji-Shen Zheng,* Shan Tang.* Chemical synthesis of a 28kDa full-length PET degrading enzyme ICCG by the removable backbone modification strategy. Bioorg. Chem. 2024, 143, 107047. (Co-corresponding)
(4) Yupeng Zheng, Baochang Zhang, Wei-Wei Shi, Xiangyu Deng, Tong-Yue Wang, Dongyang Han, Yuxiang Ren, Ziyi Yang, Yong-Kang Zhou, Jian Kuang, Zhi-Wen Wang, Shan Tang, Ji-Shen Zheng.* An enzyme-cleavable solubilizing-tag facilitates the chemical synthesis of mirror-image proteins. Angew. Chem. Int. Ed. 2024, 63, e202318897.
(5) Weiwei Shi, Tongyue Wang, Ziyi Yang, Yuxiang Ren, Dongyang Han, Yupeng Zheng, Xiangyu Deng, Shan Tang, Ji-Shen Zheng.* L-Glycodisase-cleavable natural glycans facilitate the chemical synthesis of correctly folded disulfide-bonded D-proteins. Angew. Chem. Int. Ed. 2024, 63, e202313640.
(6) Baochang Zhang, Yupeng Zheng, Guochao Chu, Xiangyu Deng, Tongyue Wang, Weiwei Shi, Yongkang Zhou, Shan Tang, Ji-Shen Zheng,* Lei Liu.* Backbone-installed split Intein-assisted ligation for the chemical synthesis of mirror-image proteins. Angew. Chem. Int. Ed. 2023, e202306270.
(7) Yongchao Wang, Shan Tang,* Capturing covalent catalytic intermediates by enzyme mutants: recent advances in methodologies and applications. ChemBioChem 2023, 24, e202300036 (ChemBioTalents 2022/2023 special collections)
(8) S. Tang,* A.T. Beattie, L. Kafkova, G. Petris, N. Huguenin-Dezot, M. Fiedler, M. Freeman, J.W. Chin,* Mechanism-based traps enable protease and hydrolase substrate discovery. Nature 2022, 602, 701-707. (First and co-corresponding)
(9) D. Cervettini, S. Tang, S.D. Fried, J.C.W. Willis, L.F.H. Funke, L.J. Colwell, J.W. Chin, Rapid Discovery and Evolution of Orthogonal Aminoacyl-tRNA Synthetase-tRNA Pairs. Nat. Biotechnol. 2020, 38, 989-999.
(10) S. Tang,# L.-J. Liang,# Y.-Y. Si,# S. Gao, J.-X. Wang, J. Liang, Z.-Q. Mei, J.-S. Zheng, L. Liu. Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer. Angew. Chem. Int. Ed.2017, 129, 13518-13522.
(11) S. Tang,# C. Zuo,# D.-L. Huang, X.-Y. Cai, L.-H. Zhang, C.-L. Tian, J.-S. Zheng, L. Liu. Chemical Synthesis of Membrane Proteins by the Removable Backbone Modification Method. Nat. Protoc. 2017, 12, 2554-2569.
(12) J.-B. Li,# S. Tang,# J.-S. Zheng, C.-L. Tian, L. Liu. Removable Backbone Modification Method for the Chemical Synthesis of Membrane Proteins. Acc. Chem. Res. 2017, 50, 1143-1153. (co-first)
(13) S. Tang,# Z. Wan,# Y. Gao,# J.-S. Zheng, J. Wang, Y.-Y. Si, X. Chen, H. Qi, L. Liu, W. Liu. Total Chemical Synthesis of Photoactivatable Proteins for Light-controlled Manipulation of Antigen–antibody Interactions. Chem. Sci. 2016, 7, 1891-1895.
(14) J. Wang,# S. Tang,# Z. Wan, Y. Gao, Y. Cao, J. Yi, Y.-Y. Si, H. Zhang, L. Liu, W. Liu. Utilization of a Photoactivatable Antigen System to Illuminate the B Cell Fingering Termination and B Cell Receptor Sorting Mechanisms in the Initiation of B Cell Activation. Proc. Nat. Acad. Sci. USA2016, 113, E558-567.
(15) S. Tang, Y.-Y. Si, Z.-P. Wang, K.-R. Mei, X. Chen, J.-Y. Cheng, J.-S. Zheng, L. Liu. An Efficient One-Pot Four-Segment Condensation Method for Protein Chemical Synthesis. Angew. Chem. Int. Ed.2015, 54, 5713-5717.
(16) X. Chen,# S. Tang,# J.-S. Zheng,# R. Zhao, Z.-P. Wang, W. Shao, H.-N. Chang, J.-Y. Cheng, H. Zhao, L. Liu, H. Qi. Chemical Synthesis of a Two-photon-activatable Chemokine and Photon-guided Lymphocyte Migration in Vivo. Nat. Commun.2015, 6, 7220
Contact Information
Address: Room K905，Annex of Life Sciences Building, West Campus, University of Science and Technology of China
Email: stang@ustc.edu.cn