Chair Professor, School of Life Sciences, Division of Life Sciences and Medicine, USTC;
Head of Division of Neural Circuits and Cognition, Hefei National Research Center for Physical Sciences at the Microscale;
Address: 443 Huangshan Street, Hefei City, Anhui 230027, P. R. China
Email: xuetian@ustc.edu.cn.
Lab Webpage: http://www.xuelab.ustc.edu.cn
Research Field of Interests:
Phototransduction, Non-image Vision, Sensory Neural Circuit, Stem Cell, Gene Editing, Photoreceptor Regeneration
Dr. Xue has long been engaged in the research of understanding physiological characteristics and signaling mechanisms of light perception, revealing neural circuits involved in this process and exploring methods for vision restoration after photoreceptor degeneration. He has published more than 50 papers and citations over 4800 times, and personal h-index is 31.
Dr. Xue revealed the photo-transduction mechanism of mammalian intrinsically photosensitive retinal ganglion cells (ipRGCs, a novel type of photoreceptors discovered two decades ago), which is crucial for understanding the kinetodynamic features of light responses in non-image-forming vision (Nature 2011). He also conducted in-depth study on light-modulated physiological functions. He depicted a novel neural pathway that mediated depressive behaviors induced by abnormal nighttime light, laying groundwork for acknowledging harmful effects of light pollution at night and exploring therapeutic interventions (Nature Neuroscience 2020). In another groundbreaking work, he achieved mammalian near-infrared vision using photoreceptor-binding up-conversion nanomaterials, reaching beyond the limits naturally set for visual perception in mammals (Cell 2019).
For translational research in ophthalmology, he developed a crispr-case9-based in vivo HDR gene editing tool, which enabled genetic repair in post-mitotic cells and rescued photoreceptor degeneration in a mouse model with an inherited visual disease (Science Advances 2019). By comparing the molecular traits of human retinae and human-origin retinal organoids, he discovered unique transcriptional factors in human retinal development (Science Advances 2020). He also optimized the differentiation conditions for stem-cell derived retinal organoids, and established a quality control system to monitor the physiological function of retinal organoids differentiated in vitro (Stem Cell Research 2019; Stem Cells 2021). He built up a single-cell transcriptome atlas of the aging human and macaque retina, which is valuable to understanding of the molecular characteristics of primate retina, as well as molecular regulation of aging progression and related diseases (NSR 2021). Moreover, he created the first nonhuman primate model for retinitis pigmentosa (RP) using adeno-associated virus (AAV)-delivered CRISPR/SaCas9 technology. This primate model is of critical importance to study the pathogenesis of RP and to evaluate potential therapeutic options in the future (Science Bulletin 2021).
Education and Positions:
01/2022–present: Assistant Principle, USTC
01/2018–05/2022: Executive Director of Division of Life Sciences and Medicine, USTC
01/2014–03/2021: Executive Dean, School of Life Sciences, Division of Life Sciences and Medicine, USTC
09/2012–present: Professor, School of Life Sciences, Division of Life Sciences and Medicine, USTC
06/2011–08/2012: Research Associate, Johns Hopkins University School of Medicine
03/2006–05/2011: Postdoctoral Fellow (with Dr. King-Wai YAU) Johns Hopkins University School of Medicine, Neuroscience
09/2000–05/2005: Ph.D., Johns Hopkins University School of Medicine, Cellular & Molecular Physiology
09/1995–06/2000: B.S., USTC, Biophysics and Neuroscience; Special Class for Gifted Young (SCGY)
Honors & Awards:
1999Zhang-Zong Zhi Outstanding Technological Student Scholarship, USTC
2001Scholarship of Cellular and Molecular Physiology (CMP), Johns Hopkins Medical School
2003American Heart Association 2003 Scientific Sessions, Selected Late-Breaking Science
2004NIH Stem Cells Scholarship at the Keystone Stem Cells Symposium, NIH
2006Best Paper Award, Circulation, American Heart Association (1 out of 1129)
2007Final list of Life Science Research Foundation Fellowship (60 out of 820)
2013 National Science Fund for Excellent Young Scholars
2014 Human Frontier Science Program (HFSP)Young Investigator Award
2019 Cell Best of 2019, Cell press (9 Best Articles which published in Cell in 2019)
2019 China’s top 10 research advances in life sciences in 2019
2019 National Science Fund for Distinguished Young Scholars
2020Significant Research Advances in Neuroscience in China
2021Zhang Xiangtong Neuroscience Young Scientist Award
2021Tan Jiazhen Life Science Innovation Award
2022First Prize of Natural Science of Anhui
Publications:
1. Yi WY#, Lu YF#, Zhong SJ#, Zhang M#*, Sun L, Dong H, Wang MD, Wei M, Xie HH, Qu HQ, Peng RM, Hong J, Yao ZQ,Tong YY,Wang W, Ma Q, Liu ZY, Ma YQ, Li SZ, Yin CH, Liu JW, Ma C, Wang XQ*,Wu Q*, and Xue T*† (2021). A single-cell transcriptome atlas of the aging human and macaque retina, National Science Review 8, nwaa179, https://doi.org/10.1093/nsr/nwaa179(# equal contributions, *co-corresponding authors，†lead contact)
2. Li L, Zhao H*, Xie H, Akhtar T, Yao Y, Cai Y, Dong K, Gu Y, Bao J, Chen J, Zhang M, Zhong K, Xu W*, Xue T*† (2021). Electrophysiological characterization of photoreceptor-like cells in human inducible pluripotent stem cell-derived retinal organoids during in vitro maturation. Stem Cells 39，959-974.
3. Li SZ#, Hu YZ#, Li YQ#, Hu M, Wang WC, Ma YQ, Cai Y, Wei M, Yao YC, Wang Y, Dong K, Gu YH, Zhao H, Bao J, Qiu ZL, Zhang M*, Hu XT*, Xue T*† (2021). Generation of nonhuman primate retinitis pigmentosa model by in situ knockout of RHO in rhesus macaque retina. Science Bulletin 66, 374-385.
4. Cheng P#, Tian X#, Tang W, Cheng J, Bao J, Wang H, Zheng S, Wang Y, Wei X, Chen T, Feng H, Xue T, Goda K, and He H. (2021). Direct control of store-operated calcium channels by ultrafast laser. Cell Res. 7, 758-772.
5. Huang X#, Huang P#, Huang L#, Hu Z, Liu X, Shen J, Xi Y, Yang Y, Fu Y, Tao Q, Lin S, Xu A, Xu F, Xue T, So KF*, Li H*, and Ren C*† (2021). A Visual Circuit Related to the Nucleus Reuniens for the Spatial-Memory-Promoting Effects of Light Treatment. Neuron 109, 347-362 e347.
6. Li X#, Zhang J#*, Li D, He C, He K, Xue T, Wan L, Zhang C, and Liu Q*†. (2021). Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory. Neuron109, 957-970 e8.
7, Liu Q#, Su LY#*, Sun C, Jiao L, Miao Y, Xu M, Luo R, Zuo X, Zhou R, Zheng P, Xiong W, Xue T, and Yao YG*† (2020). Melatonin alleviates morphine analgesic tolerance in mice by decreasing NLRP3 inflammasome activation. Redox Biol 34, 101560.
8. An K#, Zhao H#*, Miao Y, Xu Q, Li YF, Ma YQ, Shi YM, Shen JW. Meng JJ, Yao YG, Zhang Z, Chen JT, Bao J, Zhang M and Xue T*† (2020). A Circadian Rhythm-gated Subcortical Visual Circuit for Nighttime Light Induced Depressive-like Behaviors, Nat. Neurosci.23, 869-880.
9. Lu Y#, Shiau F#, Yi W#, Lu S, Wu Q, Pearson J, Kallman A, Zhong S, Hoang T, Zuo Z, Zhao F, Zhang M, Tsai N, Zhuo Y, He S, Zhang J, Stein-O’Brien G, Sherman T, Duan X, Fertig D, Goff L, Zack J, Handa J, Xue T*, Bremner R*, Blackshaw S*,Wang X* and Clark B*† (2020). Single-cell analysis of human retina identifies evolutionarily conserved and species-specific mechanisms controlling development. Dev. Cell. 53, 473-491.
10. Xie H#, Zhang W#, Zhang M#*, Akhtar T, Li Y, Yi W, Sun X, Zuo Z, Wei M, Fang X, Yao Z, Dong K, Zhong S, Liu Q, Shen Y, Wu Q, Wang X, Zhao H, Bao J, Qu K*, Xue T*† (2020). Chromatin accessibility analysis reveals regulatory dynamics of developing human retina and hiPSC derived retinal organoids. Sci. Adv. 6, eaay5247.
11. Akhtar T, Xie H, Khan MI, Zhang M*, Xue T*† (2019). Accelerated photoreceptor differentiation of hiPSC-derived retinal organoids by contact co-culture with retinal pigment epithelium, Stem Cell Res., 39, 101491.
12. Ma Y#, Bao J#*, Zhang Y#, Li Z, Zhou Z, Zhou X, Wan C, Huang L, Zhao Y, Han G*, Xue T*† (2019). Mammalian Near-Infrared Image Vision through Injectable and Self-Powered Retinal Nanoantennae. Cell 177, 243-255 e215. Cell Best of 2019, Cell press (9 Best Articles which published in Cell in 2019)
13. Cai Y#, Cheng T#, Yao Y#, Li X, Ma Y, Li L, Zhao H, Bao J, Zhang M*, Qiu Z*, Xue T*† (2019). In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway. Sci Adv 5, eaav3335.
14. Yu N, Huang L, Zhou Y*, Xue T*, Chen Z*, and Han G* (2019). Near-Infrared-Light Activatable Nanoparticles for Deep-Tissue-Penetrating Wireless Optogenetics. Adv Healthc Mater, e1801132. (review)
15. Huang L, Xi Y, Peng Y, Yang Y, Huang X, Fu Y, Tao Q, Xiao J, Yuan T, An K, Zhao H, Pu M, Xu F, Xue T, Luo M, So KF, Ren C (2019). A Visual Circuit Related to Habenula Underlies the Antidepressive Effects of Light Therapy. Neuron 102, 128-142 e128.
16. Zhu H#, Wang N#, Yao L, Chen Q, Zhang R, Qian J, Hou Y, Guo W, Fan S, Liu S, Zhao Q, Du F, Zuo X, Guo Y, Xu Y, Li J, Xue T, Zhong K, Song X, Huang G*, Xiong W* (2018). Moderate UV exposure enhances learning and memory by promoting a novel glutamate biosynthetic pathway in the brain. Cell173, 1716-1727 e1717.
17, Ye F, Huang Y, Li J, Ma Y, Xie C, Liu Z, Deng X, Wan J, Xue T, Liu W, Zhang M (2018). An unexpected INAD PDZ tandem-mediated plcbeta binding in Drosophila photo receptors. Elife 7.
18,. Deng K, Shen J, Wang W, Li M, Li H, Chen C, Zhao H, Zhang M, Xue T, Liu Q, Lui VWY, Hong B*, Lin W* (2018). Sodium chloride (NaCl) potentiates digoxin-induced anti-tumor activity in small cell lung cancer. Cancer biology & therapy 20, 52-64.
19. Deng WL, Gao ML, Lei XL, Lv JN, Zhao H, He KW, Xia XX, Li LY, Chen YC, Li YP, Pan D, Xue T, Jin, Z. B (2018). Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients. Stem Cell Rep.10,1267-1281.
20. Tang J, Qin N, Chong Y, Diao Y, Yiliguma, Wang Z, Xue T, Jiang M, Zhang J*, Zheng G* (2018). Nanowire arrays restore vision in blind mice. Nat. Commun. 9, 786.
21. Cheng J, Huang X, Liang Y, Xue T, Wang L*, Bao J* (2018). Plasticity of Light-induced Concurrent Glutamatergic and GABAergic Quantal Events in the Suprachiasmatic Nucleus. J Biol Rhythms 33, 65-75.
22. Huang DF, Wang MY, Yin W, Ma YQ, Wang H, Xue T, Ren DL*, and Hu B*†. (2018). Zebrafish Lacking Circadian Gene per2 Exhibit Visual Function Deficiency. Front Behav Neurosci 12, 53.
23. Li Q, Cui M, Yang F, Li N, Jiang B, Yu Z, Zhang D, Wang Y, Zhu X, Hu H, Li PS, Ning SL, Wang S, Qi H, Song H, He D, Lin A, Zhang J, Liu F, Zhao J, Gao L, Yi F, Xue T, Sun JP, Gong Y, Yu X(2017). A cullin 4B-RING E3 ligase complex fine-tunes pancreatic δ cell paracrine interactions. J Clin Invest. 127, 2631-2646.
24. Wang Q, Yue WWS, Jiang Z, Xue T, Kang SH, Bergles DE, Mikoshiba K, Offermanns S, Yau KW (2017). Synergistic Signaling by Light and Acetylcholine in Mouse Iris Sphincter Muscle. Curr Biol. 27, 1791-1800.
25. Liu CH, Gong Z, Liang ZL, Liu ZX, Yang F, Sun YJ, Ma ML, Wang YJ, Ji CR, Wang YH, Wang MJ, Cui FA, Lin A, Zheng WS, He DF, Qu C, Xiao P, Liu CY, Thomsen ARB, Joseph Cahill T, Kahsai AW, Yi F, Xiao KH, Xue T, Zhou Z, Yu X, Sun JP (2017). Arrestin-biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling. Nat. Commun. 8, 14335.
26. Wen M, Guo X, Sun P, Xiao L, Li J, Xiong Y, Bao J, Xue T, Zhang L, Tian C (2015). Site-specific fluorescence spectrum detection and characterization of hASIC1a channels upon toxin mambalgin-1 binding in live mammalian cells. Chem Commun (Camb) 51, 8153-6.
27. Hu QX, Dong JH, Du HB, Zhang DL, Ren HZ, Ma ML, Cai Y, Zhao TC, Yin XL, Yu X, Xue T, Xu ZG, Sun JP (2014). Constitutive Gαi Coupling Activity of VLGR1 and its Regulation by PDZD7. J Biol Chem 289, 24215-2
28. Zhong X, Gutierrez C, Xue T, Hampton C, Vergara MN, Park T, Zambidis E, Meyer J, Gamm MD, Yau KW, Canto-Soler MV (2014). Generation of three dimensional retinal tissue with functional photoreceptors from human iPSCs. Nat. Commun.5, 4047.
29. Welsbie DS, Yang Z, Ge Y, Mitchell KL, Zhou X, Martin SE, Berlinicke CA, Hackler L Jr, Fuller J, Fu J, Cao LH, Han B, Auld D, Xue T, Hirai SI, Germain L, Simard-Bisson C, Blouin R, Nguyen JV, Davis CH, Enke RA, Boye SL, Merbs SL, Marsh-Armstrong N, Hauswirth WW, Diantonio A, Nickells RW, Inglese J, Hanes J, Yau KW, Quigley HA, Zack DJ (2013). Functional genomic screening identifies dual leucine zipper kinase as a key mediator of retinal ganglion cell death. PNAS 110, 4045-50.
30. Pearson RA, Barber AC, Rizzi M, Hippert C, Xue T, West EL, Duran Y, Smith AJ, Chuang JZ, Azam SA, Luhmann UFO, Benucci A, Sung CH, Carandini M, Yau KW, Sowden JC, Ali RR (2012). Restoration of vision after transplantation of photoreceptors. Nature485, 99-103.
31. Xue T*, Do MT, Riccio A, Jiang Z, Hsieh J, Wang HC, Merbs SL, Welsbie DS, Yoshioka T, Weissgerber P, Stolz S, Flockerzi V, Freichel M, Simon MI, Clapham DE, Yau KW* (2011). Melanopsin Signaling in Mammalian Iris and Retina. Nature 479, 67-73. (Faculty of 1000: f1000.com/13360986).
32. Do MT, Kang SH, Xue T, Zhong H, Liao HW, Bergles DE, Yau KW (2009) Photon capture and signalling by melanopsin retinal ganglion cells. Nature457, 281-7. (Faculty of 1000: f1000.com/1144855).
33. Luo DG, Xue T, Yau KW (2008) How vision begins: An odyssey. PNAS 105, 9855-62 (review)
34. Fu YB#, Kefalov V#, Luo DG#, Xue T#, Yau KW (2008). Quantal noise from human red cone pigment. Nat. Neurosci. 11, 565-71
35. Xue T, Siu CW, Lieu DK, Lau CP, Tse HF, Li RA (2007). Mechanistic role of I(f) revealed by induction of ventricular automaticity by somatic gene transfer of gating-engineered pacemaker (HCN) channels. Circulation115, 1839-1850.
36. El-Kholy W, MacDonald PE, Fox JM, Bhattacharjee A, Xue T, Gao X, Zhang Y, Stieber J, Li RA, Tsushima RG, Wheeler MB. (2007) Hyperpolarization-activated cyclic nucleotide-gated channels in pancreatic beta-cells. Mol. Endocrinol.21, 753-764.
37. Tse HF, Xue T, Lau CP, Siu CW, Wang K, Zhang QY, Tomaselli GF, Akar FG, Li RA (2006). A bio-artificial sinus node constructed via in vivo gene transfer of an engineered pacemaker (HCN) channel reduces the dependence on electronic pacemaker in a sick sinus syndrome model. Circulation114, 1000-1011. Issue highlights, GROUND-BREAKING STUDY OF 2007 AHA.
38. Ardehali H, Xue T, Dong P, Machamer C (2005). Targeting of the mitochondrial membrane proteins to the cell surface for functional studies.Biochem Bioph Res Comm. 338, 1143-51.
39. Abraham MR, Henrikson CA, Tung L, Chang MG, Aon M, Xue T, Li RA, O'Rourke B, Marban E (2005). Antiarrhythmic engineering of skeletal myoblasts for cardiac transplantation. Circ Res. 97, 159-67
40. Moore JC, van Laake LW, Braam SR, Xue T, Tsang SY, Ward D, Passier R, Tertoolen LL, Li RA, Mummery CL (2005). Human embryonic stem cells: Genetic manipulation on the way to cardiac cell therapies. Reprod Toxicol. 20, 377-91.
41. Azene EM, Xue T, Marban E, Tomaselli GF, Li RA (2005). Non-equilibrium behavior of HCN channels: Insights into the role of HCN channels in native and engineered pacemakers. Cardiovasc Res. 67, 263-73.
42. Wang G#, Xue T#, Tsang SY, Wong J, Cheng L, Zhang J, Li GR, Lau CP, Li RA, Tse HF (2005). Electrophysiological properties of pluripotent human and mouse embryonic stem cells. Stem Cells 23, 1526-34.
43. Xue T#, Cho H#, Akar F#, Tsang SY, Jones S, Marbán E, Tomaselli GF, Li RA (2005). Functional Integration of Electrically Active Cardiac Derivatives from Genetically Engineered Human Embryonic Stem Cells with Quiescent Recipient Ventricular Cardiomyocytes. Insights into the Development of Cell-Based Pacemakers. Circulation 11, 111-20. Issue highlights, Best Paper Award, Circulation 2005
44. Xue T, Sato K, Kodama K, Ennis I, French RJ, Li RA (2003). Novel interactions identified between µ-conotoxin and the Na+ channel Domain I P-loop: Implications for toxin-pore binding geometry. Biophys. J.85, 2299-310.
45. Azene EM#, Xue T#, Li RA (2003). Molecular basis of the effects of potassium on heterologously-expressed pacemaker (HCN) channels. J. Physiol. 547, 349-356.
46. Henrikson CA, Xue T, Dong P, Sang D, Marbán E, Li RA (2003). Identification of a surface charged residue in the S3-S4 linker of pacemaker (HCN) channel that influences activation gating. J. Biol. Chem. 278, 13647-54.
47. Li RA, Ennis I, Xue T, Nguyen HM, Tomaselli GF, Goldin AL, Marbán E (2003). Molecular basis of isoform-specific µ-conotoxin block of cardiac, skeletal muscle and brain Na channels. J. Biol. Chem. 278, 8717-24.
48. Xue T, Li RA (2002). An external determinant in the S5-P linker of the pacemaker (HCN) channel identified by sulfhydryl modification. J. Biol. Chem.277, 46233-42.
49. Xue T, Marbán E, Li RA (2002). Dominant-negative suppression of HCN1- and HCN2-encoded pacemaker currents by an engineered HCN1 construct: Insights into structure-function relationships and multimerization. Circ. Res.90, 1267-1273.
50. Li RA, Sato K, Kodama K, Kohno T, Xue T, Tomaselli GF, Marban E. (2002). Charge conversion enables quantification of the proximity between a normally-neutral mu-conotoxin (GIIIA) site and the Na+ channel pore. FEBS Lett.511, 159-164.
Patent:
1.Xue Tian, Cai Yuan: Gene editing composition or kit used for in vivo gene therapy. ZL201811144025.3，2021.10.19，CN10926648B
2. Xue Tian, Li Shouzhen, Zhang Mei: Construction of rhesus monkey model of retinitis pigmentosa based on an in vivo gene knockout method. Patent Application No. 202010685115.4
3. Xue Tian, Li Shouzhen, Zhang Mei: A method of highly infecting macaque photoreceptor cells with shh10 serotype of adeno-associated virus. Patent Application No. 202010685517.4
4. Xue Tian, Jin Tengchuan, Zhang Mei, Cai Yuan, Tong Dali: Adeno-associated virus-mediated vaccine against SARS-CoV-2. Patent Application No. 202010903143.9
Research Support:
● Human Frontier Science Program (HFSP) Young Investigator Grants
● Major National Scientific Research Programs in the Ministry of Science and Technology (国家重大科学研究计划青年专题)
● National Natural Science Foundation for Excellent Young Scholars Grants (国自然优青)
● National Natural Science Foundation of China (Major Program)
● Chinese Academy of Sciences Pioneer Initiative (中科院先导专项)
● National Natural Science Foundation for Distinguished Young Scholars (国自然杰青)
● National Key R&D Program of China (重点研发计划)
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